Product Details
PRODUCT DESCRIPTION
Bifidobacterium lactis HH-BA68 powder contains the Bifidobacterium lactis HH-BA68 and a special protective agent. This strain was isolated from healthy infant intestine, it can survive in the acidic gastric environment and reach the small intestine and colonize the host. In where, it acts in sevearl ways to enhance health.
SPECIFICAITON
Strain name |
Bifidobacterium lactis |
Strain number |
HH-BA68 |
Potency |
500bn cfu/g |
Gene Identification |
Yes |
Deposit |
CCTCC |
Certification |
ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA |
Packing |
500g,1kg or 5kg per foil bag |
Storage |
-18℃ for long time, 2-8℃ for short time |
TECHNICAL INDICATORS
Items |
Specification |
1) Live Bifidobacterium lactis potency |
≥ 5.0×1011 CFU/g |
2) Water content |
≤8.0% |
4) Coliforms |
≤10CFU/g |
5) Moulds, Yeasts |
≤10CFU/g |
6) Pathogenic bacteria |
Negative |
7) Heavy metals |
Lead (Pb) |
≤1ppm |
Arsenic (As) |
≤0.5ppm |
RELEVANT RESEARCH
Strain Name |
Identification |
Deposit No. |
Antibiotic Susceptibility |
Acid and Bile Salt resistance |
Stability |
Quality System |
B.lactis HH-BA68 |
16S rRNA |
- |
Done |
Done |
Done |
SC/ISO9001/ISO22000/FSSC22000/HACCP/
HALAL/KOSHER/FDA
|
ACID TOLERANCE
Acid resistance of Bifidobacterium lactis HH-BA68 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Bifidobacterium lactis HH-BA68 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Bifidobacterium lactis HH-BA68 can survive at low pH conditions, which means this strain has good acid resistance.
Fig 1. Acid resistance of B.lactis HH-BA68
BILE SALT TOLERANCE
Bile salt resistance of Bifidobacterium lactis HH-BA68 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Bifidobacterium lactis HH-BA68 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Bifidobacterium lactis HH-BA68 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.
Fig 2. Bile salt resistance of B.lactis HH-BA68
STORAGE STABILITY
Bifidobacterium lactis HH-BA68 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Bifidobacterium lactis HH-BA68 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Bifidobacterium lactis HH-BA68 powder was 4.40×1011CFU/g. After a storage period of 24 months, the potency of the powder was 3.60×1011CFU/g. It means that this strain can remain stable for long time if stored at low temperature.
Fig 3. Storage stability of B.lactis HH-BA68
HEALTH BENEFITS
1.Inhibit pathogen colonization
The aim of this study was evaluation of antagonistic effect of B.lactis HH-BA68 against colonization by pathogens. The results showed(Table 1) that B.lactis HH-BA68 is critical in providing resistance against colonization by four pathogenic microorganisms.
|
S.aureus |
C.difficile |
S.typhimurium |
L.monocytogenes |
Colonization inhibition rate(%) |
48.0±3.6 |
27.8±6.4 |
5.2±3.8 |
25.7±8.0 |
Colonization replacement rate(%) |
25.7±7.5 |
56.7±7.4 |
7.4±2.8 |
43.8±7.3 |
Table 1. Pothogen colonization inhibitory ability of B.lactis HH-BA68
2.Inhibit pathogen growth
The aim of this study was evaluation of antagonistic effect of B.lactis HH-BA68 against four pathogens. Antimicrobial effects of isolates were evaluated by disc diffusion test on plates with different pathogens. Growth inhibition zones of pathogens and B.lactis HH-BA68 inhibitory ability were assessed after incubation of all agar media at 37℃ for 24h. The results showed (Table 2) that B.lactis HH-BA68 had relatively strong activity against tested pathogens (with inhibition zone diameter> 15mm).
|
B.cereus |
C.difficile |
E.coli |
L.monocytogenes |
Inhibition zone’s diameter/mm |
26.9±2.9 |
24.5±1.4 |
26.7±2.3 |
36.0±4.5 |
Table 2. B.lactis HH-BA68 inhibitory ability
APPLICATIONS
Fermented milk; Dietary supplements
Functional foods (Beverages, Bakeries, meal replacement)
PACKING AND DELIVERY
COMPANY IMAGE
http://www.probioway.com/